Sentence examples for preparations primed from inspiring English sources

In all other preparations, primed substrates were incubated for 4 h with laminin (10 μg/ml for CGNs, 2 μg/ml for sensory neurons) mixed where appropriate with myelin or CSPG (100 μg/ml and 5 μg/ml respectively).

One that suggests combat readiness, primed forces and perhaps paranoia too?

Generally for an equal quantity of cDNA input, transcripts of the same gene were more abundant in random-primed cDNA preparations compared to oligo (dT -primed preparations, as manifestedT -primedrlier Cq value for the former compreparations laster.

The resulting rRNA-depleted RNA samples were then used to prepare random-primed cDNA libraries using the Illumina Tru-Seq RNA Sample Preparation Kit (Illumina, San Diego, CA) according to the manufacturer's recommendations.

Hence our RNA-seq data reflect a bias towards the 3'-end mainly attributed to the application of oligo-dT primed cDNA preparation.

I come refreshed, prepared and perfectly primed to solve every problem on the agenda".

Poly-A RNA eluted from the magnetic beads was fragmented and primed with random hexamers in preparation for cDNA synthesis.

This explains why it was detected with our sample preparation using oligo-dT-primed cDNA synthesis.

Preparation of a random-primed and normalized cDNA for pyrosequencing was done with 100 μg of pooled total RNA from liver and heart (Vertis, Freising, Germany).

Further sequence data were generated by random-primed reverse transcription of purified dsRNA preparations and cloning into pGEM-T Easy plasmid (Promega Corporation, USA).

We use these results to examine the effects of library preparation procedures, specifically, normalized versus non-normalized and random versus oligo-dT primed libraries.