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Similar experiments were performed using recordings of summed action potential activity of air-borne sound receptors, with the preparations placed in the nocturnal rainforest and receiving conspecific calls at a sound pressure level either 10 dB or 20 dB above the hearing threshold.
Yolks were separated from eggs, and pooled to provide 150 g and 300 g egg yolk preparations, placed in plastic bags and diluted 1 1 with tap water, and then refrigerated until required.
Similar(58)
Flattened cochlear preparations were placed in a recording chamber mounted on an upright microscope (Axioskop2 FX plus, Carl Zeiss, Germany).
The isolated neural preparations were placed in an orientation so that the rise of the platform corresponded to a head down tilt in the intact animal.
Aortic preparations were placed in chambers, kept at 37°C and bubbled continuously with 95% O2 – 5% CO2 in Krebs Bicarbonate Buffer (KBF) (pH 7.4).
The preparations were placed in a custom pacing and imaging chamber and voltage mapping of spontaneous cardiac electrical activity was performed using this chamber and a CCD (CardioCCD-SMQ, RedShirtImaging, Decatur, GA) mounted on a Nikon TE2000 inverted microscope.
After curing of the synthetic resin, the preparation was placed in a screw clamp to ensure adequate fixation for the preparation of the glenoid.
The preparation was placed in an electrophysiological setup and tilted such that the antennal lobes were visible through the microscope.
The preparation was placed in a horizontal perfusion trough [4 mm (width) × 5 mm (depth) × 30 mm (length)] and superfused with Tyrode's solution (28).
SR preparation was placed in a Teflon chamber equipped with a calcium-selective microelectrode (WPI, Aston, UK) to assess calcium-uptake activity.
The hippocampal preparation was placed in a custom-made submerged recording chamber lined with a nylon mesh, and firmly stabilized by carefully placing several lead weights at both septal and temporal poles of the hippocampal preparation.
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