Sentence examples for preparations overnight from inspiring English sources

The membranes were incubated with the diluted antibody preparations overnight at 4°C.

Primary antibodies were diluted in PBST with 1% NGS and incubated with the preparations overnight at 4°C.

For whole cell preparation, overnight precultures of strain SDM were inoculated into fresh GYSC liquid medium with 10% inoculum and the cultures were incubated at 50°C and 100 rpm.

In three experiments, the number of morphologically distinct CFs in RL cell preparations, after overnight incubation with 1F5, was 0.8 1.9% of the cell number.

Make special preparations for overnight guests.

Here, both the PGN preparation treated with buffer only AND the PGN preparation treated overnight with LYS1 lack immunogenic activity in the respective supernatant.

For total RNA sample preparation, after overnight growth, M1 cells were inoculated to an initial OD600nm of 0.08 0.1 in MM supplemented with respective carbon sources (0.4% lactate or 100 μl of β-myrcene) and cultures diluted again to OD600nm of 0.08 0.1 when OD600nm of 0.5 was reached to synchronize cell growth.

Preparations were incubated overnight at 4 °C with primary antibodies (Table 1) and next day washed in PBS, followed by incubation with Alexa 488- or Alexa 568-conjugated secondary antibodies (Invitrogen) for 1 h.

After blocking unspecific binding, cell preparations were incubated overnight with primary A2BAR antibody (1 : 200 v/v) followed by incubation with respective secondary antibody (1 : 500 v/v) diluted in PBS with 5% serum.

Preparations were incubated overnight at room temperature with rabbit antibodies against human caveolin-1 (Santa Cruz; 1 200 for whole mounts, 1 1000 for sections) followed by 1 hour in Cy3 conjugated anti-rabbit IgG (1 100, Jackson Immunoresearch Laboratories).

After blocking for 10 min with goat serum, the first antibody, diluted in PBS, was added (Group A received rabbit anti-mouse (RAM) FasL, Group B received RAM IL-10, and Groups C and D received RAM FasL and IL-10); preparations were incubated overnight at 4°C.