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Herein we study this detrimental effect in detail using two different preparations of the model enzyme subtilisin Carlsberg.
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The model was then submitted to a series of energy minimizations before the final preparation of the model was scored and written to an output database.
Lessons learned in the preparation of the MoDEL database, by our group, leaded to the generation of a new set of tools, MDMoby and MDWeb 91 that try to cover both aspects of the problem.
Furthermore, preparation of the model adducts (3 and 4) allowed for facile single- and two-photon spectroscopic characterization, which more closely resembles the bioconjugate than that of the amine-reactive probe (conjugate precursor).
For preparation of the model system for the IRAS and QMS experiments, a Pt(111) single crystal (MaTecK GmbH) was cleaned by several cycles of Ar+ sputtering (E = 1.5 keV, 8 × 10 5 mbar Ar, 300 K) annealing (1200 K) and oxidation (5 × 10 7 mbar O2, 700 K) in vacuum.
Furthermore, different methods for preparation of the simulation models are represented.
However, it would be possible to perform post-mortem MR imaging in most large hospitals and these images could be transmitted easily to a specialist centre for subsequent analysis and preparation of the models.
After the preparation of the models, the minipigs were cared for over 8 months at a facility specializing in the care of domestic pigs.
GR participated in performing the surgical preparation of this model and has made substantial contributions to the design of this model.
Marcelo Lufin provided valuable assistance in the preparation of the econometric model.
The preparation of the language model does not demand much manual work.
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