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Normal skin fibroblast cultures CRL-21277, ATCC) were used for large-scale preparations of normal procollagen.
To identify the specific cell types that express BORIS isoforms in adult testis, we carried out RNA in situ hybridization using fixed preparations of normal human testis.
Paraffin-embedded preparations of normal skin, nevi, primary and metastases of malignant melanomas were screened for CTGF protein expression by the avidin-biotin complex (ABC) method (DAKO-LSAB2-Kit, DAKO, Hamburg, Germany).
For further verification, we performed hybridizations of H3K4me3 ChIPs from samples V425, V432, V441, SW480 and two independent preparations of normal colon mucosa to NimbleGen 385K promoter arrays and repeated the data analysis.
An additional 98 primary cultures of primary human tumor cells (PCPTCs) from different tumor types, and four preparations of normal peripheral blood mononuclear cells (PBMC), detailed in Table 2, were used to determine the activity spectrum of VLX40 and, for comparison, six standard cytotoxic drugs chosen to represent different mechanistic classes.
Data from 63 separate preparations of normal bronchial epithelial cell cultures indicated that basal level of phosphorylated ERK was consistently low (7.5 ± 5.6 % of OSM level; mean ± SD) and the basal levels of phosphorylated STAT3 and STAT1 were generally low to non-detectable.
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The test program involved the preparation of normal and high strength concretes made out of recycled waste tire rubber.
(ii) Preparation of Normal Pooled Plasma (for Normal Control and as Diluents).
Heads and internal organs were already removed during the preparation of normal MEFs, cells but the spinal cord could still be a source for cells with neurogenic potential.
For the in vitro evaluation, the influence of the GT seedcake preparations on normal human dermal fibroblast proliferation was assessed using the MTT test and the wound scratch assay.
As controls, these genes were also analyzed in three independent preparations of purified normal colon crypts.
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