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Near homogenous preparations of native complexes are obtained in sufficient amounts to perform biochemical assays and characterize their components.
Making well-defined preparations of native ICs with which to study their therapeutic and vaccination potential is impractical.
Bornstein, Kang, and Piez [ 37] claimed that trypsin is less specific than pepsin and may not attack the iV-terminal region of many preparations of native collagen.
Of note, treatment at 37°C of control preparations of native ER (human recombinant ER, MCF-7 cells) resulted in similar behaviour.
This study also found that some commercial preparations of native SEB were comprised almost entirely of nicked protein while preparations from other vendors showed no evidence of internal cleavage [ 9].
Preparations of native single (undocked) hemichannel layers isolated from mammalian cells have not been documented, most likely because they are too dispersed in the plasma membrane and do not survive the detergent enrichment.
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Moreover, preparation of native PrP from mammalian cells and tissues, as well as recombinant PrP from bacterial cells, involves time-consuming purification steps.
Horm collagen is a preparation of native collagen fibrils from equine tendon which mainly contains predominantly type I collagen [22], [23].
Preparation of native or fluorophore-labeled biomolecules was performed as described in Johansson et al.
Currently, most H. pylori vaccines in animal models have utilised whole-cell preparation of native or recombinant proteins from the bacterium, together with mucosal adjuvant.
In Drosophila, the rapid syncytial division cycles of early embryogenesis result in a very high physiological mitotic index approaching 50%, which allows a very efficient preparation of native mitotic chromosomes.
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