Sentence examples for preparations obtained using from inspiring English sources

Table 2 Antimicrobial activity of the Natto peptide from various Natto preparations obtained using different soybeans and B. subtilis strains Natto preparation lot No. B. subtilis strain used for starter MIC (μg/mL) S. pneumoniae R6 B. subtilis AHU 1708T 160 S63 64 1281670 H2008 16 180 S12008 16 200 S200 8 8 300 S12005 3200200

Cells were harvested and chromosomal preparations obtained using standard protocols.

Electron micrographs of the proteoliposome preparations obtained using SDS and DDGly mediated reconstitution.

Similar results were obtained with four other preparations obtained using the same protocol and in two preparations in which the dye was trapped within the t-tubules before skinning (not shown).

Following the manufacturer's protocols, total RNA preparations were obtained using Purelink a Trizol RNA isolation and purification kit (Invitrogen, USA).

DNA preparations were obtained using commercially available purification kits (Sigma).

cDNA preparations were obtained using Superscript III and random primers (Invitrogen, California).

Chromosome preparations were obtained using a modification of conventional techniques explained in detail in supporting information (Text S2) [38].

For Triton X-100 extraction experiments, nuclear preparations were obtained using the NucBuster Nuclear Protein Extraction Kit (Novagen, Darmstadt, Germany) according to the manufacturer's manual except for the addition of an extraction step of the nuclear pellet in extraction buffer +0.5% Triton X-100.

Plasmid DNA preparations were obtained using the High Pure Plasmid Kit (Roche).

Photomicrographs of the preparations were obtained using computer-amplified image analysis and an optical microscope (Olympus BX53; Olympus, Tokio, Japan).