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It seems surprising that effective resaturation occurs also for apolactoferrin preparations obtained at low pH 2.0 and 3.0.

The enantioselectivities of both preparations obtained at different pH and incubation times remained essentially constant (within a 15% error) over a wide pH range (from pH 6.5 to 8.5), but it decreased slightly at the extreme pH's.

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Western blot analysis using markers for plasma membrane (Gas1p), microsomes (Wbp1p), lipid particles (Ayr1p) and mitochondria (Por1p) revealed that all preparations were obtained at high quality and purity (Table 4) in agreement with previously published results [35].

The RNA and DNA preparations obtained from both extraction processes were pooled as a total nucleic acid stock and stored at −20°C.

Cartilage pellet preparations obtained from chondrogenic differentiation were fixed in 4% PAF for 20 min and stored in PBS at 4°C until processing.

Cells were harvested and chromosomal preparations obtained using standard protocols.

The supernatant of field strain 34086b bacteriophage preparation, obtained after centrifugation at 20,000 ×  g, was serially diluted in Frey's medium supplemented with ß-NAD.

Fifty μl of dialyzed bacteriophage preparation obtained by centrifugation at 145,000 ×  g was treated with 0.5 units each of DNase and RNase for 30 min at 37°C, then 20 units of proteinase K (BRL) and 1% SDS for 1 h at 65°C.

Although a potassium hydroxide (KOH) preparation was not obtained at baseline, the discharge grew P. lilacinus in pure culture.

Preparations of R3230AC mammary tumours, obtained at designated times after a single dose of Photofrin II, displayed a time-dependent photosensitivity.

The reduced alveolar area is also evident in whole mount preparations from WT and mutant females obtained at the same time points).

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