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It may also be noted that DRM preparations observed by using freeze fracture technique in the electron microscope show that rafts are bilayer structures, although it is not known whether the lipid asymmetry is preserved during the isolation of DRM [43].
After fixation in formaldehyde 4%, cells were incubated for 90 min with a mouse monoclonal anti-p65 antibody (2 µg/ml, Santa Cruz) and FITC-goat anti-mouse IgG (Jackson laboratories) for 30 min. Nuclei were labelled using TOPRO-3 (0.04 µg/ml) and cell preparations observed using confocal microscopy.
Indeed, variability in basal release rates could account for some of the variability across preparations observed here.
Because all of the NRs are secreted protein preparations, observed Bmax values were comparable with those calculated from their molecular weight.
The exclusion of participants with chronic illnesses may have accounted for the low use of herbal medicine preparations observed in our study.
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In a recent study, Scheidegger et al. [35] showed that, with certain types of RF preparation, the observed APT in tumors at 3.5 ppm, at 3 T, could be contributed by asymmetrical MTC effects.
Since mature conditional ezrin−/− T cells exhibit defects in migration and adhesion (MHS and JKB, manuscript in preparation), the observed cortical thinning may be associated with improper movement of ezrin−/− thymocytes within the thymus.
Radiation did not cause changes in haemodynamics in these preparations, thus the observed changes in leucocyte rolling and adhesion could not be accounted for by haemodynamic factors.
The influence of the sample preparation on the observed dependence of glass transition temperature (Tg) with thickness in polystyrene (PS) thin films has been investigated.
We now describe the criteria for "spot" definition in the subsection headed "Preparation of the observed intensities".
Since sample variations or differences in sample preparations may influence any observed difference in enzyme efficiency [27], we used a well tested primer pair and viral RNA standard, the Armored HIV RNA [28], [29], thereby eliminating potential variation due to template sources.
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