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For the studies of stimulation of immune mediators it is necessary to have the fimbrial preparations free of endotoxin.
Thus, the purpose of the present study was to evaluate and compare the in vitro effects of ginger and rosemary extracts obtained by supercritical fluid extraction, a technology that renders preparations free of toxic organic solvents, using liposomes, Pluronic-F-68 and DMSO as vehicles.
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This method is relatively quick and provides a microvessel preparation free of contamination by other brain tissue.
Conventional therapy was compared to incobotulinumtoxinA (Xeomin®; Merz Pharmaceuticals GmbH, Frankfurt/M, Germany), a BoNT/A preparation free of complexing proteins [13].
Preliminary results with incobotulinumtoxinA, a preparation free of complexing/accessory proteins, have indicated a low antigenicity.
IncobotulinumtoxinA is a preparation free of complexing proteins with a high specific biological activity.
The new BoNT/A preparation free of complexing proteins seems to be such a candidate.
Evaluation of antigenicity of incobotulinumtoxinA, a botulinum neurotoxin type A preparation free of complexing proteins for the treatment of cervical dystonia.
Further purification over a gelfiltration column of the antibody containing eluate resulted in an estimated 95% pure preparation free of light chain homodimers, with a total estimated product recovery of 22%.
This indicates the purity of the astrocyte preparation free of contamination with microglia and is consistent with reports from other groups using the same isolation procedure, in which astrocyte cultures contained >98% astrocytes (Morgan et al., 1995).
To determine whether zymosan-induced systemic inflammation alters peripheral chemoreceptor activity, we recorded CSN activity in a superfused in vitro carotid body/CSN preparation free of possible confounding factors present in vivo such as metabolic acidosis and circulating inflammatory mediators.
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