The present trial compared the safety and immunogenicity of PfCS102 synthetic peptide 282-383 itsits GMP preparation formulated in three doses with Montanide ISA 720 or AS02A.
L-BLP25 is a lyophilized preparation, formulated to contain 241 μg of BLP25 lipopeptide and 128 μg of immunoadjuvant monophosphoryl lipid A in a 5 mL glass vial.
The first preparation, formulated according to the process described by Schwarz et al 20 (the same method as that used in the production of Tissucol) had a higher ratio of clottable to total protein than did the second, which was formulated according to the method described by Martinowitz and Bal 21 (used in the production of Quixil), but at a much lower fibronectin to fibrinogen ratio.
Synthetic surfactant preparations were formulated by mixing synthetic lipids (SL), consisting of 16∶10∶6∶1∶2 (weight ratio) DPPC∶POPC∶POPG∶POPE∶cholesterol, with 1.5 mol% S-MB, MB, SP-B(1 8), or native pig SP-B.
VA preparations are formulated in sodium chloride (NaCl 0.9%) isotonic solution as 5 mg/ml vials.
VLP preparations were formulated with 15% trehalose to 100 ng Gag/100 μl PBS then stored at -70°C.
The oil-based antigen preparations were formulated as water-in-oil (w/o), where the water phase (containing bacterial antigens) was dispersed into an oil phase (continuous phase containing emulsifiers and stabilizers).
Several pathogens express antigens with an amino acid sequence homologous to the HERV-K-MEL epitope, but either the relevant proteins are not used to arrange the respective vaccines (e.g. tetanus toxoid and acellular pertussis vaccine), or most non-viable preparations are formulated to induce humoral response rather than cellular immunity [ 38].
A traditional Chinese medicine (TCM) preparation was formulated from orange peel (Pericarpium Citri Reticulatae), hawthorn (Crataegus pinnatifida), astragalus (Astragalus membranaceus (Fisch).
Combined UV-C plus aqueous ozone (1 ppm) provided 3.13 ± 0.47 log CFU/fruit Salmonella reduction which was significantly lower (p < 0.05) compared to the rest of the combination treatments; whereas the treatment of UV-C followed by immersion in a novel antimicrobial preparation 'HEN', formulated mixing hydrogen peroxide, EDTA and nisin provided the best log reduction (4.71 ± 0.25 log CFU/fruit).
