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To validate the microarray results, the remaining twelve RNA samples (3 experimental and 3 control preparations for each of the two tissues) were used to prepare independent cDNA samples with the Superscript 1st Strand Synthesis Kit (Invitrogen).
Malusa's human encounters are mostly pedestrian, and he spends too much time recounting preparations for each of his journeys, including several days of negotiation to retrieve his satellite phone, seized by customs officials in Cairo.
As a veteran, he has fine insights into the attitudes of his comrades, and can clearly explain the gear, training and preparations for each mission, as well as the dangers.
He did, however, acknowledge some gay-specific challenges, likening his preparations for each year's banned demonstrations to a James Bond film: "We always have to use new tactics, we always have to invent some new strategies to bypass the bureaucrats".
The analysis was carried out in two separate preparations for each functionalized motif in triplicate for each sample.
Further, each experiment was performed using a minimum of 3 different preparations for each plasmid reporter construct.
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To minimize systematic bias in library preparation, for each genome we prepared two paired-end DNA libraries with a read length of 90 bp and insert sizes of 475 504 bp (Table S1).
He read widely and prepared his sermons with diligence, reckoning on an hour's preparation for each minute in the pulpit.
Arrange friendlies with local clubs in preparation for each season's crucial matches.
In preparation for each day's explorations, the Rover drivers sit in front of twin computer monitors.
Lee's seemingly casual, laid-back style belied her meticulous preparation for each performance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com