For different concentration of Fe3+ cations, the yield was also 74% (mean value) in most cases (7 out of 9 preparations) (Figure 8).
Unspliced sense transcripts with a long 3'UTR were not detected in polysomal preparations (Figure 7).
Culture with 250 µM-P was cytotoxic for the four preparations (Figure 5a).
The level of inhibition was essentially sustained in the IgG preparations (Figure 3B&C).
We also measured mitochondrial Ca2+ uptake, with strikingly different dynamics observed between preparations (Figure 2C).
The SAA1 and SAA2 specific primers did not amplify any product in any of the monocyte preparations (Figure 4).
As shown by qPCR, CEsHUT induced the expression of IL-1β mRNA in the 3 monocyte preparations (Figure 3A).
PCR with SAA 3 specific primers amplified a product with the expected size of 328 bp in all four monocyte preparations (Figure 4).
Quantification of blots indicated marked differences in the relative abundance of complex I, II, III and IV between preparations (Figure 4A).
Ponceau S staining confirmed that the total amount of protein loaded onto the gel was similar in embryonic and adult membrane preparations (Figure 2A, lower panel).
