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It was shown that the immobilization procedure used for the sensor preparation ensured the highly repeatable ammonium sensing with the acceptable sensitivity.
Thus, the same preparation ensured that the two-photon imaging should be stable through the artificial dura and that multiple recordings could be made.
The volume of each insoluble fraction loaded onto a 10% gel was pre-adjusted so that the amount of MOMP was equal in each preparation, ensuring that equal numbers of chlamydial organisms were examined [29].
This sample preparation ensured the alignment of the bacteria along the sample plane and allowed observation of live bacteria.
Second, the slice preparation ensures that all synapses are local, so MEA recordings are influenced only by the local circuit dynamics and not by input from other areas.
