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As placebo an identical enema preparation devoid of the active substance, was used.
A concurrent dissolution was performed with preparation devoid of drug to record the interference from excipients, if any.
RNA preparations were devoid of genomic DNA contamination as standard PCR analysis failed to detect amplicons using the primers and conditions of the RT-PCR reactions described below (data not shown).
Such preparations are devoid of functional cell membranes and a functional sarcoplasmatic reticulum, while the myofilament lattice of the myofibrils remains structurally intact.
PCR reactions using RNA samples as templates never showed a PCR band and always showed a PCR band with cDNA controls (data not shown), indicating that our RNA preparations were devoid of contamination by genomic DNA.
Electron microscopic analysis showed that this preparation is devoid of microfibrillar components.
This suggests that the H. muridarum LPS preparation was devoid of peptidoglycan muropeptides, which might otherwise induce NOD1 signaling in m-ICcl2 cells.
The flagellin preparation was devoid of LPS contamination, as indicated by the Limulus assay (< 0.0003 μg LPS/μg flagellin).
Analysis of other organelle markers revealed the preparation was devoid of early endosomes and golgi, but retained ER and lysosomal markers (Additional File 1: Figure S1).
The observed strong depletion (>50%) of the mitochondrial signature lipids, PG and CL (see Fig. 2C), in flagella compared to whole cells was expected, as the flagella preparation was devoid of cell remnants.
However, acute slice preparations are largely devoid of the ongoing network activity and functionally significant DA tone, which can influence neuronal responses [11] [14].
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