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In addition, the inflammatory cytokines were not exceptionally concentrated at higher levels in PRP preparations, and no strong positive correlation between WBC counts and pro-inflammatory cytokine levels was observed in PRP preparations (data not shown).
Similarly, different potencies have also been noted with commercially available LF preparations (data not shown).
This finding has been replicated in our hands across experiments within and between vector preparations (data not shown).
Early stages of sperm development appear normal by standard phase-contrast microscopy of testis squash preparations (data not shown).
The differential expression of each of these miRNAs was validated in two independent RNA preparations (data not shown).
Other cytokine receptors such as IL-2Rα and IL-7Rα could not be detected on Dex preparations (data not shown).
Similar(32)
IDE is the only insulin-degrading activity in this preparation (data not shown).
This increase was accompanied by variability in ERK1/2 activation, which returned to baseline levels 4 5 h following slice preparation (data not shown).
Northern analysis of viral genomic RNA isolated from lysed HIV-1 indicated that a co-purifying nuclease was present in the sucrose-cushion purified virus preparation (data not shown).
We have found that cutting slices in a high-sucrose ACSF with a reduced calcium/magnesium ratio enhanced the probability of recording cholinergic neurons in our slice preparation (data not shown).
Exposure of individual FFPE sections to light and air had a negative influence on RNA quality, but storage of entire FFPE blocks either in the open or protected from air and light did not influence the effect of storage time on RNA quality, as long as the uppermost section was discarded at each time point, and sections not directly exposed to air were used for RNA preparation (data not shown).
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