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With this assay we observed that our treatment (5 h incubation in 0.1 M NaOH) at 56 °C efficiently inactivated LPS in all polysaccharide preparations below immune-stimulatory levels.
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The injection of a high dose of 100 µg LT/rat did not reduce the TTD for LF-HMA preparations below 50 min (Table 2).
For all worm preparations (below), worms were concentrated into a volume of 200 μl to which an equal volume of TRI reagent (Sigma Genosys Ltd., UK) was added, which was then snap frozen in liquid nitrogen and subsequently stored at -80°C until required.
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The protein content of each antigen preparation was below 0.5 mg/ml, measured by absorption at 280 nm (Spectrophotometer NanoDrop 1000, Thermo Fisher Scientific, Bonn, Germany).
The colours are unevenly distributed on the surface, thus revealing large areas of the white preparation below.
The colours were unevenly distributed on the surface, leaving large areas of the white preparation below to shine through.
A recent modification in bowel preparation is the addition of oral contrast agents (see 'Bowel preparation' below).
Thirdly, to exclude the possibility that low levels of single-stranded nucleic acids present in the hybrid preparation below the sensitivity of PAGE analysis could represent a source of contamination, the FPLC process was repeated using "mock" hybridisation reactions in which one of the constituent oligonucleotides (ssRNA60 or ssDNA60) was omitted.
Endotoxin contamination was monitored with PyroGene-rFC Endotoxin Detection System (Lonza) and was found in all preparations to be below 0.5 endotoxin units/µg protein.
Only ClC-3 could be detected by western blot analysis in comparable amounts in control CHO, wt ClC-7+Ostm1 and G213R ClC-7+Ostm1 membranes (Fig. 2D), indicating that ClC-3 is not responsible for the altered transport activities found in the different preparations (see below).
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