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The same DNA preparations as used for rep-PCR were also used to amplify dnaK genes using the primers and protocol described previously (Stępkowski et al. 2003; Martens et al. 2007).
DNA templates were the same preparations as used in aCGH.
Eight differentially expressed miRNAs were selected for the validation in which the same RNA preparations, as used in the microarray, were subjected to q-RT PCR.
Total RNA from the same RNA preparations as used for microarray analysis was reverse transcribed using random primers and Superscript III (Invitrogen), in accordance with the manufacturer's instructions.
The presented study investigated the impact of intracoronary perfusion of crystalloidal and colloidal infusion preparations as used in the clinical setting in an isolated guinea-pig heart model.
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To confirm the alterations observed by microarray analysis we performed qRT-PCR using oligo dT) primed cDNA synthesis on the same RNA preparation as used for microarrays.
Costs were based on a manual preparation system as used in 'Sentinel site' laboratories undertaking the HPV testing.
However it was still possible to collect spectroscopic data without further sample preparation such as using a microtome to provide a smooth surface.
The samples were depleted using the same procedure as used for preparation of the peptidomic samples.
Both preparation and insufflation are performed as used in current clinical practice in our institution.
DMSO diluted to equivalent concentrations as used in the 200 nM 8-azacytosine or 100 μg/mL RG-108 preparations was used as vehicle controls.
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