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The manager is expected to select Didier Drogba in Costa's stead against the champions as the London club seek to extend their lead at the top, though his preparations are further complicated by knocks sustained this week to members of his squad and the proposed departures of André Schürrle and Mohamed Salah.
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Burgess' preparations were further hindered when he was routinely swapped from inside centre to No.6 at English club Bath and back to No.12 by England.
Fast indoor courts have always been the world number two's least-favourite surface and his preparations were further disrupted when he called the trainer to tape his fingers before play had even begun.
The endotoxin preparations were further purified and quantified as described [18].
The presence and quality of exosomes in our preparations was further verified by transmission electron microscopy (TEM), and by determining acetylcholinesterase (AChE) activity and expression of Alix protein.
The proteolysis reactions were terminated by adding PMSF to 5 mM and incubated for an additional 5 min. The preparations were further mixed with 5 volumes of boiling buffer (1% SDS and 0.1 M Tris, pH 8.9), digested with PNGase F and transferred onto nitrocellulose and probed with mAb 3F4.
These preparations were further incubated as indicated above.
These preparations were further incubated and revealed as above to obtain the MMCs.
High quality total RNA preparations were further treated with DNase I (Promega).
Affinity purified antibody preparations were further tested to determine their reactivities and specificities.
These preparations were further incubated at 30°C for five days on the plate shaker at 300 rpm.
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