M/z = Mass to Charge ratio, Tertiles of the intensities per peak were determined in the controls, separately for day of preparation, applied to cases prepared on the same day and afterwards combined in three categories.
We also tested the commercial preparation Bicep II Magnum [reported as 33% atrazine, 0.7% atrazine-related products, 26.1% technical-grade S-metolachlor (18.57% S-metolachlor, 2.5% R-metolachlor), 40.2% inert ingredients], a commercial preparation applied to the field twice in 2001.
Standard chromosome preparations were applied to peripheral lymphocyte cultures [57] of male individuals of human (Homo sapiens, HSA), chimpanzee (Pan troglodytes; PTR), bonobo (Pan paniscus, PPA), gorilla (Gorilla gorilla, GGO), Bornean and Sumatran orang-utan (Pongo pygmaeus, PPY), white-cheeked crested gibbon (Nomascus leucogenys; NLE), and rhesus macaque (Macaca mulatta; MMU).
1 μL of these preparations was applied to the MALDI plate, and allowed to dry at rt.
The preparations were applied to LS magnetic MACS columns, washed with 3 ml PBS 3 times and eluted with 4.5 ml PBS.
Briefly, drops of phage preparations were applied to pioloform-carbon-coated, 400-mesh copper grids (Plano GmbH, Wetzlar, Germany), incubated for 10 min and fixed with 2.5 % aqueous glutaraldehyde (Taap Laboratories, Aldermaston, United Kingdom) for 1 min.
For the semi-critical articles high level disinfection, formaldehyde in alcoholic at 8.0% v/v or aqueous at 10.0% v/v preparations are applied to an 18-hour exposure.
For this purpose, VLP preparations were applied to 400-mesh carbon-coated grids, negatively stained with 1.5% uranyl acetate and then examined at a nominal magnification of 50 000 with a Jeol 1010 electron microscope.
When the same IgG preparations were applied to cerebellar granular cells in culture, however, the electrophysiological responses became less dependent on P/Q-type voltage-gated calcium channels, with an increase in the contributions of other voltage-gated calcium channels (Supplementary material B213).
Three microliters of the DNA preparations were applied to the Rickettsia genus specific quantitative real-time PCRs (qPCRs) targeting the 17-kDa antigen gene (Rick17b) (8 ) and gltA (9 ), as well as the R. parkeri species specific qPCR (Rpark), as described (8 ).
