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During the recordings, neurons were maintained in hypothalamic slice preparations and data analyses were performed as previously described Frazao et al (2013).
Data for wild-type mNPC were derived from four independent preparations, and data for AhR-deficient mNPCs were obtained from two different preparations.
Sample preparations and data analysis for 3′-seq were performed as previously reported [ 22] using total RNA extracted from 8-week-old C57BL/6 male mouse tissue, including testis, liver, brain and skeletal muscle.
The functional gene group approach adapted in this study relies on grouping genes according to similar cellular function on the basis of extensive lab experiments (whole-synapse analyses and solubilized preparations) and data mining.
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S.O.S. assisted with sample preparation and data collection.
X.L. performed all immunoblotting studies and MS/MS sample preparation and data analysis.
K.Y., H.P., and A.C.B. helped with the CRISPR-Cas9 library preparation and data analysis.
Metabolomics sample preparation and data analysis was performed by O.D.K.M. and T.Z.
The staff provide consultation on experimental design, library preparation, and data analysis.
The case studies and the sections on data preparation and data quality reflect the real-world challenges in the effective use of predictive analytics.
The field has since evolved to incorporate new technologies for faster and highly automated sample preparation and data collection.
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