Before conducting the final analyses several preparation analyses were conducted.
ST provided toxicological expertise, help with the implementation of the study, data preparation, analyses, and the writing of the manuscript.
AØ has received speaker's fees from GSK, BI, and Pfizer, and honorarium from advisory boards from BI. ED: study preparation and execution, analyses, paper writing; AØ: study execution, analyses, paper writing; HM: study preparation, analyses, paper writing.
Sample preparation, analyses by the ethyl chloroformate NEFA method and GC, were performed by applying methods described in detail by Amer et al.. Quantification of ethyl chloroformate-NEFA esters was obtained using external calibration curves for each NEFA.
A proportion of each sample was removed before slide preparation, analysed on a Dako CyAn ADP and data evaluated using Summit 4.3 Software.
Contribution: LG contributed to study design, sample preparation, data analyses, and manuscript preparation.
HN participated in the conception of the project, experimental design, sample preparation, data analyses, and manuscript preparation.
N = 4 different independent RNA preparations and analyses.
TWL and CCW carried out the sample preparations, material analyses, and characterization tests.
TWL carried out the sample preparations, material analyses, and characterization tests.
