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Such non-invasive visualization in living preparations allowed us to acquire high-magnification, sequential optical sections (z-stacks) that were used to generate high-resolution anatomical, volumetric images of embryos.
Such non-invasive visualization of chromatin in living preparations allowed us to acquire high-magnification sequential optical sections (z-stacks) that can be used to generate high-resolution anatomical volumetric (3-dimensional) images with details of interphase chromatin in addition to mitotic chromosomes and fragmenting nuclei.
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Isolated papillary muscle preparations allow us to properly control preload and afterload and thus analyze intrinsic myocardial function.
Such sample preparation allowed us to avoid the use of fixative media and paraffin having IR spectral signatures that can mask absorption bands of biological components [ 21].
Again, the use of the larval zebrafish preparation allowed us to record intracellularly from Purkinje neurons and extracellularly from motor neurons simultaneously.
The depletion procedure used in the third protocol of sample preparation allowed us to estimate inter-individual variation of von Willebrand factor concentration (1.15 fM, CV = 59%%) in six samples.
Visually guided patch-clamp experiments in the slice preparation allowed us to target only L5 pyramidal neurons.
This preparation allowed us to stimulate small areas of the ventral basal nucleus of the thalamus, while monitoring the response of layer 4 at both the multi-neuronal level, using calcium imaging, and at the single cell level, with targeted whole-cell recordings (Figure 1).
This preparation allowed us to study the effect of the test substance on both rate and force of spontaneous atrial contractions.
This enzyme and our method of library preparation allowed us to sample, per population, an estimated 85.5k restriction cut sites (with 10 reads or more) distributed throughout the genome on 5900 scaffolds (approximately 173 cut sites/Mbp) and sampling approximately 42k variable loci.
The zebrafish larval preparation allows us to perform intracellular recordings of membrane potential in the absence of anesthesia.
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