Sentence examples similar to preparation were washed from inspiring English sources

After 90 seconds, the preparation was washed with Tyrode solution.

The preparation was washed twice with 150 µl PBS/0.5% BSA, incubated with 50 µl secondary antibody diluted in PBS/0.5% BSA for 30 min at 4°C, washed twice in PBS/BSA and re-suspended in 500 µl of PBS/BSA.

The immobilised preparation was washed with deionised water followed by enzyme assay buffer.

After the incubation period, the culture medium was aspirated, and the preparation was washed with PBS.

The preparation was washed with fresh toluene, and MTBE and was finally air-dried at room temperature.

The preparation was washed with Drosophila saline several times to remove debris and any soluble agents released during the dissection.

After this period the preparation was washed with an excess of 10 mM sodium phosphate at pH 7 and assayed.

Finally, the immobilized preparation was washed with 10 mM sodium phosphate at pH 7. 2 g of activated agarose were incubated with 20 ml of 100 mM sodium bicarbonate pH 10.05 containing 8 U (at 65°C) of soluble NOX.

After incubation for 10 min, the preparation was washed with ice cold HL3 for 10 15 min. This leads to an intraterminal OGB-1 concentration of approximately 50 µM (Müller and Davis, 2012).

Finally, the immobilized preparation was washed with 10 mM sodium phosphate at pH 7. A volume of 20 mL of 10 mM sodium phosphate at pH 7 containing 8 U (at 65°C) of NOX was mixed with 2 g of metal-IDA support.

The skull preparations were washed with SIF at room temperature for 30 min and then mounted in a humid chamber at 37 °C.