Sentence examples for preparation were treated from inspiring English sources

Nuclei from 1/5 of each preparation were treated with four ul of RNAse A, 10 ug/ul, (Qiagen) and used for Micrococcal Nuclease (Takara) digestion for 6 minutes (final concentration 0.2 U/ul) in digestion buffer (16 mM Tris-Cl, pH 7.6, 50 mM NaCl, 2.5 mM CaCl2, 0.01 mM PMSF and EDTA-free protease inhibitor cocktail (Roche) and stopped with 10 mM EDTA.

Mice used for the cell preparation were treated according to the guidelines of the IACUC Institutional Animal Care and Use Committeee) of the Medical College of Georgia to minimize pain or discomfort.

Each RNA preparation was treated with DNase I according to the manufacturer's instructions.

RNA preparation was treated for DNA removal with deoxiribonuclease I (Sigma) and cDNA was obtained from 1 μg of RNA by using the iScript cDNA Synthesis Kit (Biorad).

The RNA preparation was treated with 10 U of DNase1 for 30 min at 37°C.

cDNA insert preparation was treated with Proteinase K, cleaned, and digested with the appropriate restriction enzymes.

The response option "no specific preparation" was treated as an exclusive category.

Two of these fragments, the 15 and a 19 kDa peptides, could be visualized when our full-length Ets1 preparation was treated with trypsin.

Before synthesis of first-strand cDNA, the RNA preparation was treated with RNase-free DNase I (Invitrogen) to eliminate any residual genomic DNA.

Each RNA preparation was treated with 2 units of DNase I (New England Biolabs) at 37°C for 30 min followed by inactivation at 70°C for 10 min.

In these studies the preparation was treated with ATP to loosen the actin meshwork surrounding newly formed phagosomes and thus improve purity (Gotthardt et al., 2002; 2002).