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To control for inter-culture and inter-animal variability, approximately 50% of neurons from a given neuronal preparation were tested with antagonist or vehicle, respectively.
Differences in quality of bowel preparation were tested with the χ test.
For determination of minimal inhibitory concentrations (MIC) different concentrations of the preparation were tested by the method described above.
Two methods of fragment shearing during library preparation were tested to determine whether that step of the methodology could affect coverage patterns: physical shearing with the Covaris system and enzymatic shearing with the Ion Shear Plus reagent.
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In the longest clinical safety study ever conducted with CR, the same CR preparation was tested for one year and endometrial safety assessed by histological evaluation of the tissue.
This RSV preparation was tested negative for both endotoxin and mycoplasma.
Yield and purity of the protein preparation was tested by coomassie staining and western blot of SDS-PAGE gels.
Each IgG preparation was tested for specificity against target antigens using Western blotting.
Each preparation was tested for the presence of VLPs by electron microscopy.
RNA integrity for each RNA preparation was tested using an Agilent 2100 Bioanalyzer (Agilent technologies).
RNA integrity of each RNA preparation was tested using an Agilent 2100 Bioanalyzer (Agilent technologies).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com