Sentence examples for preparation were loaded from inspiring English sources

Briefly, equal quantities (8  μg) of the same preparation were loaded on two 1D-PAGE gels and submitted to electrophoresis.

The optimum conditions for the preparation were loading amount of 2 wt%, calcination at 700 °C in air followed by reduction at 400 °C in 5%H2.

After dialysis, the protein preparation was loaded onto anion exchange UNOsphere Q (Bio-Rad), and cation exchange UNOsphere S (Bio-Rad) columns (column volume, 15 mL).

The dialyzed crude preparation was loaded onto a preactivated DEAE-cellulose column (15 × 2 cm) equilibrated with 50 mM phosphate buffer (pH 7.8).

In the first step, the crude preparation was loaded onto a Q Sepharose FF column (anion exchanger XK 26/20, GE Healthcare) and the proteins were eluted with a linear gradient of 0-0.7 M NaCl in 10 mM sodium acetate buffer (pH 5.5) at a flow rate of 6 ml min-1.

Total RNA was quantified by spectrophotometric analysis (λ = 260 nm), and 5 µg of RNA of each preparation was loaded onto a 1% agarose gel and separated in 10 mM sodium phosphate buffer as described previously [20].

The preparation was loaded onto 1 mL HiTrap NHS-activated HP coupled with purified recombinant CT.

Once equilibrated, 1 mL of the total protein (TF) preparation was loaded onto the LCA column.

The preparation was loaded into the IPG strip holder, and care was taken not to produce any bubbles.

The virion-enriched preparation was loaded on a 10%% polyacrylamide gel and electrophoresed until all proteins entered the gel.

Subsequently, the entire cDNA preparation was loaded to the centre of the column and centrifuged at 700 g for 5 min.