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Two milligrams of each standard preparation were dissolved in 10 mL of methanol.
The dried residues from each preparation were dissolved in DMSO and sterilized by passing through a 0.22 μm membrane filter for the biological assays.
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The final RNA preparation was dissolved in RNase free water and stored in −80°C until use.
Concentrated OPP preparation was dissolved and diluted serially with buffer and added in cumulatively, directly to the bath.
All RNA preparations were dissolved in RNase free water and were then treated by DNase as previously described [29].
Precipitated P. falciparum protein preparations were dissolved in digestion buffer, digested with trypsin and LysC, and analyzed by LC/LC/MS/MS according to published protocols [22], [23].
For mass analyses purified rhamnolipid preparations were dissolved in 50% acetonitrile-water and characterized by electrospray-ionization-mass-spectrometry (ESI-MS) in the negative ionization modus.
Such raft preparations were dissolved in 6% SDS-RIPA buffer (150 mM NaCl, 25 mM Tris HCl pH 7.4, 5 mM EDTA, 0.5% Na-DOC and 0.5% NP40).
The RNA preparations were dissolved in 40 μl RNase free water.
The pharmaceutical preparations were dissolved according to instructions from the manufacturer, the other drugs were dissolved in dimetylsulfoxid (DMSO; Sigma-Aldrich, Stockholm, Sweden) or dimethylacetamide (DMA; Sigma-Aldrich, Stockholm, Sweden) and stored frozen in −70°C for maximum three months.
Crude RNA preparations were dissolved in 300 μl DEPC-treated water by agitation with sterile pipette tips at 68°C for 20 30 min. The samples were cooled down on ice, and RNA pools were precipitated with 300 μl 4.0 M LiCl (prepared in 20 mM Tris-HCl pH = 7.5 buffer, also containing 10 mM EDTA) and were left at -20°C overnight.
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