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The standard solutions that were previously prepared as a bulk preparation were diluted into murine DNA from untreated E- and P-selectin-deficient scid mice.
10 to 100 µl of the colanic acid preparation were diluted to 1 ml with distilled water, and mixed with 4.5 ml of H2SO4/H2O (6∶1 v/v).
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The DNA preparation was diluted with ddH2O (3:100), and 1 μL of the diluted DNA was used for PCR analysis.
Briefly, the lentiviral preparation was diluted tenfold serially in PBS (from undiluted to a dilution of 10-5).
The NLC/PI-Dac preparation was diluted at 10%% in PBS and incubated at 37 °C.
For mouse injections the final rEA preparation was diluted in 0.1% human serum albumin (HSA), in 0.9% saline (USP grade, Abbott Labs, Abbott Park, IL) and filter-sterilized.
The preparation was diluted in sterile distilled water.
The preparation was diluted with water (1 : 100 v/v) before sample preparation.
The concentration of mitochondrial preparation was diluted to 40 μg/ml and used for staining.
Enzyme preparation was diluted in each buffer (1 : 2, v/v) and incubated for 24 h at 10°C.
100 μL of cell preparation was diluted with 400 μL of PBS and analyzed in the FACS.
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