Sentence examples for preparation were analyzed from inspiring English sources
Forty-eight hours later, the supernatants were harvested, concentrated and purified as above described, and equal volumes of each preparation were analyzed by anti-Nef Western blot.
The resultant cRNAs were resuspended in RNA resuspension buffer (5 mM Hepes, 0.1 mM EDTA, pH 7.5) and samples of each preparation were analyzed by agarose-formaldehyde gel electrophoresis.
Risk factors associated with poor bowel preparation were analyzed and indentified as follows: age 65 years old and above, lower education level (no formal education or primary education only), in-patient status, waiting time for colonoscopy appointment beyond 16 weeks and non-compliance to bowel preparation instructions (Table 2).
The samples were prepared in triplicate, and each cell preparation was analyzed twice.
Following an overnight digestion with 2% pepsin at pH 4 in 0.1 M sodium acetate, the preparation was analyzed by size-exclusion high-performance liquid chromatography (SE-HPLC) and then dialyzed against phosphate-buffered saline (PBS) over the course of 4 days, three changes per day.
Again each preparation was analyzed against the common reference RNA.
The homogeneity of the preparation was analyzed by SDS-PAGE.
Each preparation was analyzed 10 times to obtain mean and standard deviation [41].
The purity of the preparation was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the protein concentration determined by measuring the absorbance at 280 nm.
PrPSc purified with the guanidinium protocol from a representative ovine scrapie preparation was analyzed by 2D electrophoresis and then probed with the monoclonal antibody 6H4 and the lectins.
Briefly, 1 mL of infected Vero cells supernatant was recovered after 11 days of infection (first observation of the cytopathic effect), ultracentrifugated (250 000 rpm at 4°C for 3 h in a Beckman SW41 rotor ultracentrifuge) and then incubated with 10 µg of magnetic beads for 10 min. Magnetic beads were then recovered by using a magnet and the preparation was analyzed by western blot.
