Exact(30)
For these experiments, NPD water was used to prepare PBS, which was then used to make a PEG/DMSO solution; as a control preparation we used PEG prepared in DI based PBS.
Thus, for sample preparation we used a novel multistep physicochemical approach.
For the ball crater preparation we used a steel ball with a diameter of 20 mm and a diamond paste with a grain size 0.25 μm.
To investigate the role of posterior brain regions related to task-relevant stimulus processing in task preparation, we used a cued task-switching paradigm in which a pre-cue informed participants about the upcoming task on a trial: face discrimination or number comparison.
Having established an acceptable specificity for anti-ΔP antibody preparation, we used it in direct immunoblots of rat brain extracts.
For image analysis and figure preparation we used the LSM Image Browser, Volocity, ImageJ and Adobe Photoshop and Illustrator.
Similar(30)
For the preparation, we use a cold-wall CVD reactor especially designed for handling samples up to a size of a 4" wafer.
To preserve the simplicity of preparation, we use the CSP method for deposition of both components in the composite.
To determine the amount of active hAID in our preparations, we used a previously published active site titration method13, 44.
Due to the potential heterogeneity of various MEFs preparations, we used several independent isolate of MEFs derived from wt and p53 KO embryos for quantitative real time RT-PCR (QRT-PCR) analysis.
To further assess the purity of the immunopurified preparations, we used MALDI-TOF mass spectrometry, and found that the only identifiable protein was PrP (probability-based MOWSE Mascot score: 85; matched peptides: 13; sequence coverage: 30%).
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