As a preparation, we identified barriers of implementing this checklist, using a questionnaire based on an instrument of the Netherlands Organization for Applied Scientific Research TNOO), which is designed to survey determinants that influence the uptake of an innovation in health care organizations [ 12, 13].
By examining different fractions generated by the crushing centrifugation of embryos during extract preparation, we identified a slower-migrating species of importin α enriched in the lipid/membrane fraction at stage 8, thereby decreasing importin α levels in the cytoplasmic fraction.
Analyzing the components in our defined medium preparations, we identified three major sources of xeno material: BSA as a component of various media formulations, geltrex (which was used as a substrate in several stages of the process) and PA6-CM.
Using stringent selection criteria of at least 10-fold higher expression in three distinct preparations, we identified 154 RPE signature genes, which were validated by qRT-PCR analysis in RPE and in an independent set of 11 tissues.
By mimicking hemolysis caused by improper blood collection or preparation we have identified a list of 162 miRNAs that are most likely to be affected by hemolysis in a clinical setting.
Indeed, we have evidence that Fltp regulates cell division of intestinal stem cells (Böttcher and Lickert, in preparation), suggesting that we identified a novel molecule with brought implication for ciliary disease and stem cell-mediated tissue homeostasis.
37 We identified four studies evaluating preparation for and/or receipt of surgery.
We identified a human vSMC preparation that showed enhanced proliferation in response to high glucose media.
We used gene expression data of G978 and its wild-type parent IR64 (R. Mauleon et al, manuscript in preparation) to identify genes that were differentially expressed at 24 and 48 h after inoculation.
Whole brain morphology data will also be compared to morphology data generated in an acute slice preparation to identify features common to both datasets (Available on our cell types database http://portal.brain-map.org/).
