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A 30 µl aliquot from the final preparation was frozen and forwarded for RPM-Flu analysis.
The membrane preparation was frozen in liquid nitrogen and stored at −80 °C.
The clear supernatant fluid with its active lysin obtained during the final stage of lysin preparation was frozen at −20°C [ 6].
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Cells to be used for BBM vesicle (BBMV) preparation were frozen immediately in liquid nitrogen and stored at −80°C until required.
An alternative to fixation in SEM sample preparation is freezing.
Then, PRP and PPP preparations were frozen and stored at −20 °C until further used (usually within 2 weeks).
Smooth muscle preparations were frozen by immersion in ice-cold acetone containing 5 mM NaF (-80°C).
The SUV preparation was freeze-dried and resuspended in PBS-containing A β (10 mg) and thoroughly mixed.
One part of the tumor was placed in neutral buffered formalin for paraffin block preparation, and the other part was frozen for preparation of cryo-cut sections.
After preparation, entrees were frozen and delivered to participants on a weekly basis.
The NLC and NLC-Dac preparations were freeze-dried to obtain dried product and then subjected to PXRD analysis.
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