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Each media preparation was filtered, and dialyzed fetal bovine serum (dFBS) was added to a final volume of 10%, Glutamax was added to a final concentration of 1X, and Penicillin/Streptomycin was also added to a final concentration of 1X.
Following endotoxin removal, the final preparation was filtered through a sterile filter.
After the preparation was filtered, the M. charantia L. extract was stored at 4°C.
The liposome preparation was filtered with a membrane filter (0.22 μm pore size) and used.
The preparation was filtered, placed in a sterilised 3.0 L glass container, and stored at 4°C before use.
The preparation was filtered through 20 μm nylon and stained with prodidium iodide to a final concentration of 50 ppm.
Similar(51)
Preparations were filtered to remove debris and were resuspended in RPMI.
Preparations were filtered to remove debris and were resuspended in RPMI with 10% FCS.
After enzymatic treatment, skeletal preparations were filtered through a 40-μm cell strainer and undigested bone material was discarded.
The strom and epi preparations were filtered with 40-μm Falcon cell strainers before labeling for MACS.
At these two time intervals, the preparations were filtered through a 0.8/0.45 μm filter and the target compounds were measured in the filtrates.
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