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The amount of library required for template preparation was calculated using the template dilution factor calculation described in the protocol.
The contractile response for each preparation was calculated as stress (Newtons/m), normalized to the cross-sectional area of the longitudinal smooth muscle layer as previously described [ 9].
The relative antibacterial potency of a given preparation was calculated by comparing its zone of inhibition with that of the standard antibiotic tetracycline.
The minimum concentration of the sugar in the final reaction mixture, which completely inhibited 32 hemagglutination units of the protein preparation, was calculated.
The average daily intake of energy (kcal/day) and 44 nutrients (μg, mg or g/day), adjusted for losses due to food preparation, was calculated using the data base from the National Food Administration in Sweden and the software MATs (Rudans Lättdata, Västerås, Sweden).
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The porosity of pSi microparticle preparations was calculated by gravimetric analysis [42], and the microparticle thickness and pore sizes were calculated via SEM.
The active site concentration of the various RT preparations was calculated as described below.
The relative concentration of the various cDNA preparations was calculated with the 2−ΔCt method; the Q-RT PCRs were run with 1 µl of cDNA or 10-fold dilutions with the above conditions.
The amount of apoAI in the preparations was calculated from the [C]apoAI-specific activity.
A semiquantitative histological score for each of the slides was performed and the mean score of the five preparations was calculated.
MT concentration in the recombinant preparations was calculated from the acidic ICP sulphur measurements, thus assuming the only contribution to their S content was that made by the HpCuMT and HpCdMT peptides.
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