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The pH of the preparation was adjusted when adjusted to 7.4 by adding an appropriate volume of 10 N NaOH.
A spore preparation was adjusted to a 0.5 McFarland standard with a Microscan turbidity meter, resulting in a 1 x 10 CFU spores/mL suspension.
The preparation was adjusted to pH 7.2, DMSO (v/v)(Sigma) was added to 10% followed by sterile filtration of the PEG solution through a 0.45 μm filter (Millipore).
It was found that all respondents could evaluate the sixteen full-profile choice tasks in a reasonable amount of time but the phrasing of the attributes sensitivity, specificity and preparation was adjusted slightly to increase the understanding of the questions.
166.6 μg/mL × 1% / 100 = 1.666 μg/mL 1.666 μg/mL × 3 mL/10 mL = 0.5 μg/mL In the event that a dialysate sample's determined concentration falls outside of the system's calibration curve of 10 ppm (i.e., greater than 20% iron transfer), the sample's preparation was adjusted so that the final determined concentration would fall within the system's calibration range of 0 to 10 ppm elemental iron.
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Cell lysates were normalized by protein content, and corresponding exosome preparations were adjusted accordingly.
All thermostable enzyme preparations were adjusted to pH 6.0 and treated at 60°C for 2 hours to inactivate the background T. reesei enzymes.
To remove the less thermostable enzymes produced by the host strain T. reesei, the two xylanase preparations were adjusted to pH 6.0 and were treated at 60°C for 2 hours.
The sample preparation workflow was adjusted and refined in the levels of deproteinization, incubation, and reconstitution; the general pipeline is illustrated in Figure 1.
After completion of the preparation procedure, the ventilation was adjusted to yield a PaCO2 between 38 and 41 torr (5.0 5.5 kPa).
To conform to the human daily dose of the preparation, rat daily dose was adjusted according to the ratio of human and rat body surface areas.
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