With both issues in mind, we evaluated the use of 3 kits prior to library preparation to remove inconsistencies between samples.
Total RNA was treated with RQ1 DNAse (Promega, Madison, WI) at a concentration of 1 unit/µg RNA preparation to remove genomic DNA.
By incorporating an additional purification step during library preparation to remove excess unligated adapters, this effect can be mitigated.
The N-HOPG was annealed at 1000 K for 30 min in the preparation chamber to remove the initially adsorbed gas before the XPS and XAS measurements were performed.
10% citric acid solution was used to finish the chemomechanical preparation in order to remove the smear layer formed during the root canal preparation [ 18].
For biochemical characterization of the β-glucosidase, the SP188 commercial preparation was desalted to remove the ingredients present in the industrial formulation.
Because many of the polymers containing amino acids were rather hydrophilic and could absorb atmospheric moisture during preparations, samples were heated to remove the absorbed water, cooled, and reheated again at a heating/cooling rate of 20°C/min.
In previous experiments, the enzyme preparations were heat treated in order to remove the minor side activities present in the preparations.
The resultant chondrocyte preparations were washed again with PBS to remove the trypsin, and were then incubated for about 8 hours in Dulbecco's modified Eagle's medium (DMEM; Gibco) supplemented with 0.1% type-II collagenase (Worthington Biochemical Co., Lakewood, OH, USA), 10% heat-inactivated FBS (Equitech-Bio, Inc., Kerrville, TX, USA) and antibiotics.
Standard blank was also used to remove the errors during standards preparation; it consists of reagent water and all reagents that are normally in contact with standard preparation.
