Single read libraries were prepared using the TruSeq sample preparation low throughput protocol (gel method) following manufacturer instructions (Illumina, San Diego, CA, USA).
Thin-layer chromatography is a very popular technique for the assessment of TCMs in Chinese Pharmacopoeia, due to the advantages of lower cost, less rigorous sample preparation, higher throughput, and easier visualization (Sowa and Subbaiah 2004).
All eight RNAseq library preparations were performed according to manufacturer's recommendations (Illumina Truseq RNA sample Preparation Low Throughput protocol).
RNA-seq libraries were constructed with the TruSeq RNA sample preparation (low-throughput protocol) kit from Illumina.
While this manuscript was in preparation, high-throughput sequencing also identified osa-miR1425 buthesese was not described further [ 10, 11].
This method of protein immobilization could be utilized for preparation of high throughput products requiring structurally ordered molecular interfaces, in addition to many other applications.
This method enables the mild, facile, and high-throughput preparation of DOX conjugates that retain the basic C3′-nitrogen, a pre-requisite for topoisomerase II inhibition.
This method is suitable for high-throughput preparation of transcription templates [ 22].
The high-throughput preparation of high-quality supercoiled DNA for cell-free protein expression was performed as described [ 19].
Using the 'split-primer' PCR method for the high-throughput preparation of transcription templates and the wheat germ cell-free system, we constructed protein libraries including 35 E2s and 204 RING E3s.
