In anaesthetised cat preparations, low threshold electrical stimulation of the second or third cervical nerve evokes activity in the hypoglossal and abdominal nerves, whilst either exciting or inhibiting sympathetic activity in the splanchnic nerve (Bolton et al. 1998).
The implementation of the algorithm is a part of the BioMinerva framework (SM Kielbasa, in preparation), the chosen threshold of false discovery rate is the default 5%.
Expression values were determined from standard curves generated from each RNA preparation, plotting cycle threshold values against log quantity.
For the skin nerve preparation experiments, Von Frey thresholds were expressed as the geometric mean (in mN) together with their 95% confidence limits in brackets or as the median with 1st and 3rd quartiles.
The activation sizes determined by various criteria are the most variable measure in this preparation, but adaptive statistical thresholding appears to yield the most stable and reproducible maps.
The real-time PCR experiments were performed twice, with both independent total RNA and cDNA preparations by the comparative threshold cycle method.
The relative mRNA expression levels of COL1A1, COL1A2 and Smad7 were normalized against the GAPDH gene from the same RNA preparations, using a comparative threshold cycle method.
Endotoxin levels in the profilin preparation were below the detection threshold (<0.1 ng/µg) as assessed by the limulus assay.
Ongoing work with simulated data confirms the impact of the pruning threshold (Walters & Lubke, in preparation).
Calves — lactate threshold training.
