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Since the sample requirement is small and the method allows for analysis of membrane potential even if some mitochondria are disrupted during sample preparation, this method could even be applied to studies in which minimal amounts of samples are available (e.g., human tissue).
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However, sample preparation for this method is difficult.
An experimental set-up is described for the preparation by this method of homogeneous layers of catalysts deposited on the two sides of a desired number of plates.
Figure 1A shows a preparation using this method.
Third, ssDNA ligase can be used to ligate Illumina adapters directly to ssDNA during library preparation, however, this method has not been tested on viral DNA [ 30].
Sample preparation by this method also removed the pilus from the complex, the empty T4P machinery was nonetheless still clearly visible.
Either wash and condition your hair as usual, or wet your hair and pat it dry in preparation for this method.
Such IgY preparations by this method have been used in other human studies [11], [31], and they contain low levels of egg cholesterols and triglycerides [18].
Antibody conjugate preparations following this method yielded conjugates with an average of 3.3 triazole-linked products per antibody.
The intra- and inter-molecular MQC signals can also be detected separately with specific preparation periods, but this method is sensitive to coupling constants.
On-target capture efficiency is primarily a result of the library preparation process and this method has a high reproducibility.
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