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To test the absence of protease contamination in the PA28-αβ preparation, the substrate MBP102 129 was digested in the usual digestion solution without 20S proteasomes for 60 mins and no detectable degradation of the substrate was observed.
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In four out of five gland preparations, the substrate for endogenous NO formation, L-arginine (0.1 mmol/L), decreased the AP ratio.
In addition, no special preparation of the substrate is needed.
These were believed to occur during the preparation of the substrate or fabrication process.
In general, surface preparation of the substrate concrete is considered essential to achieve a durable repair.
The preparation of the substrate comprises cleaning, development of a rough surface and sometimes preheating to guarantee mechanical anchorage at microwelding sites.
The yield of thin nanotubes as determined by scanning electron microscopy (SEM) is strongly dependent on the precise CVD process and the preparation of the substrate.
Hence, it is envisaged that the use of UHPFC concomitant with appropriate surface preparation of the substrate should be able to provide effective and durable concrete repair.
The advantages of this method are without externally applying stress and special sample preparation, and the substrate effect can be avoided.
The in situ activation of a SERS substrate is performed simultaneously with the analytical detection of a probe molecule, controlling the process related to the preparation of the substrate and performing the analytical measurement in real time.
Furthermore, the appropriate preparation of the substrate is crucial for the production of maximized yield of Pleurotus mushrooms (Choi et al. 2009; Obodai and Johnson 2002; Soliman 2011).
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