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During preparation the sample was held at an angle of 57° which led to a more homogeneous wetting.
Cross-section preparation: the sample is embedded in CEM4000 Lightfix (methylmethacrylate light curing embedding resin supplied by Cloeren Technology GmbH) and polished, with the assistance of a sample holder, on micromesh sheets up to grade 12000.
Assuming that it costs ∼250 dollars for target capture and library preparation, the sample preparation cost for a project with 1000 individuals would be $250,000.
Since well-characterized bipartite synapses were also rare in our preparation, the sample number was too small for estimating transmission probability.
After membrane preparation the sample was heated to T = 37°C for 15 min to equilibrate the bilayer in the mixed phase.
During library preparation the sample amplification can result in duplicate reads for the same original transcript which is particularly relevant if an unknown amount of RNA is accidentally lost before amplification.
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The preparation of the sample and the Swagelok cell was entirely completed in an Argon glove box.
Slight activation may be due to contact activation during the preparation of the sample.
For sample preparation, the etching bath sample is diluted before analysis due to the high-HF concentration.
F.C. contribute in the preparation of the samples and the acquisition of the SEM images, moreover F.C. prepared the lamellas for the TEM images via FIB.
M.S. collected the biomass samples and assisted in the preparation of the samples.
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