To generate high-titer preparations, the recombinant vectors were amplified in HEK-293 cells and purified over three successive cesium chloride gradients.
Our present work provided an efficient approach for the preparation of the recombinant INSL6 peptide and its analogues for further studies of this important peptide.
For large scale preparation of the recombinant protein, 1 L of bacterial culture was induced under the optimum condition.
We thank Patricia Fagnant for the preparation of the recombinant smooth muscle myosin proteins.
Heterologous expression and preparation of the recombinant protein was carried out according to [ 35].
This method eliminates the background signal caused by the binding of antibodies against traces of E. coli proteins which remained in the preparation of the recombinant antigens.
Here, we developed a new method for the preparation of the recombinant human MLCP complex and determined the molecular and cellular basis of inhibitory phosphorylation.
This span of variation was noted from four different individual preparation of the recombinant protein (45, 520; 47, 320; 49, 220; 56, 103 units mg−1 protein).
The preparation of the recombinant TacGINS protein will be described elsewhere (Ogino et al).. Briefly, the gene encoding the protein (Ta1042) was cloned into the pET21a vector, and was overexpressed in E. coli BL21 codonPlus™ (DE3 -RIL cells.
To increase antibody specificity and assay sensitivity, we purified the HSP60 antigens in their native form, leaving out denaturants in the preparation of the recombinant protein or as part of the elution buffer [ 32].
