To accomplish this we used an in vitro preparation of the mouse where the spinal cord was isolated (T5-cauda equina) with one hind limb left attached.
We demonstrate the advantages of this approach with a number of deconvolution experiments performed both on artificially blurred and noisy test images, and on real confocal images taken within an in vitro preparation of the mouse hearing organ.
The preparation is the mouse olfactory bulb [14].
We have employed in our study an ex vivo explant preparation of the mouse retina which constitutes a useful model system for addressing neurotransmitter regulation of microglial morphology and behavior.
More details about sample preparation of the mouse brain tissue section and purchased standards including lipids, peptides, and proteins can be found in the Supporting Information.
The authors thank Dr Hideki Fujii for the preparation of the mouse liver fibrosis tissue samples and Drs Hiroyuki Motoyama, Le Thi Thanh Thuy, and Tohru Komiya for valuable comments on this study.
The single isolated mature skeletal muscle fiber preparation from the mouse FDB muscle has been previously used with ROS-sensitive fluorescent probes (Pye et al., 2007; Palomero et al., 2008; Vasilaki et al., 2010) and was applied here.
It was shown in the late 1970s that a critical step in cumulus expansion is the deposition of a HA matrix and that the synthesis of this glycosaminoglycan was very important in the preparation of the mouse oocyte cumulus cell complex for normal ovulation (Eppig, 1979).
This study was designed to investigate the acute effects of PlTX and ouabain, also an Na+/K+-ATPase blocker, on the mammalian peripheral nervous system using an ex vivo electrophysiological preparation: the isolated mouse sciatic nerve.
Their characteristic morphology makes them readily distinguishable from the endothelial cells in IB4-labeled specimen, where whole cells may be observed, such as in whole-mount preparations of the mouse retina and in thick tissue sections of the CNS (Fig 1A,D and data not shown).
