So, the preparations can be envisaged as coating of protein molecules over clusters of nanoparticles. Figure 3 Hydrodynamic diameter of the various ECCN preparations suspended in n -propanol as measured by dynamic light scattering.
Experiments were performed in preparations suspended between two L-shaped stainless steel hooks in a 10 ml organ bath containing Mac Ewen solution.
Mice were used in in-vivo prokinetic and laxative studies while in-vitro experiments were conducted on isolated tissues of rabbit and guinea-pig gut preparations suspended in a tissue bath to measure isotonic contractions.
Briefly, for the AChE assay, 15 µl of the exosomal preparation was suspended in 100 µl of PBS and incubated with acetylthiocholine (1.25 mM) and 5,5'-dithio-bis- 2-nitrobenzoic acid) (0.1 mM) in a final volume of 1 ml.
The separated tumour cell preparation was suspended in IMDM (Gibco BRL, Rockville, MD, USA) including 10% FBS.
Finally, the preparation was suspended from an electronic balance (BG 025, Mark-10, NY, USA) and connected to a ventilator (T-Bird AVS III, Thermo Respiratory Group, CA, USA).
Figure 6 Atomic Force Microscopy images of (A) EPRP (B) ECCN 3. In all the cases (EPRP, ECCN 1-ECCN 5) when the preparations were suspended back in the aqueous buffers, ≥91% activity could be recovered (Table 1).
In experiments in which LA-induced [Ca2+]m efflux and peroxynitrite generation were determined in the absence of Ca2+ refilling, the mitochondrial preparations were suspended in MB1 buffer and diluted with nine volumes of HKG-EGTA buffer (containing 200 µM EGTA instead of 1 mM CaCl2 as in HKG), then labeled with X-rhod-1 and DCF and measured as described above.
Final RNA preparations were suspended in RNase-free water.
Otherwise, membrane preparations were suspended in the aforementioned buffer without sucrose.
