Sentence examples for preparation strain from inspiring English sources
For inoculum preparation, strain FGL3-6 of the pathogen, maintained on potato dextrose agar medium, was incubated in CM medium (complete medium with casein acids hydrolysate 10 g/l, casein enzyme hydrolysate 10 g/l, yeast extract 16 g/l, and lactose 20 g/l) on a rotary shaker (150 r/min, 26°C) for three days.
For bacterial inoculum preparation, strains were grown to exponential phase in Mueller-Hinton broth (Merck, Germany) at 37°C for 24 h and adjusted by diluting fresh cultures to turbidity equivalent to 0.5 McFarland scale (approximately 2 × 10 CFU mL−1) and then diluted until 1 × 10 CFU mL−1, as described by Clinical and Laboratory Standards Institute [ 22].
For culture supernatant protein preparations, strains were cultured in Sauton supplemented with 0.5% glycerol.
For inoculums preparations, strains stored at −72°C in glycerol were inoculated into Luria broth (LB) for overnight growth.
For DNA preparations, strains were grown overnight in 45 mL of Brain-Heart-Infusion (BHI) broth at 37°C.
For the sample preparation, each strain was inoculated from the frozen stock into 10 mL of M9 medium for the preculture.
The sample preparation and strain analysis was based on a recent work investigating the influence of lattice strain on surface electronic and chemical states of La0.8Sr0.2CoO3−δ.
Agarose gel electrophoresis of plasmid DNA preparation from strain UVA1 showed three bands: two extrachromosomal DNA elements at 9.5 kb and at >10 kb and one corresponding to chromosomal DNA, respectively).
An alternative method for preparation of strain-specific antibody that can be used for SRID potency assay is described.
prOVag is a preparation containing strains of three lactic acid bacteria: L.fermentum 57A, L.plantarum 57B, and L.gasseri 57C.
The synbiotic preparation included strains of Bifidobacterium longum W11, one of the most representative species of gut microbiota, and oligosaccharides, which exert a positive effect on intestinal motility and favor the development of bifidobacteria in the gut lumen [ 85].
