The method was a convenient way to produce homogenous preparations of spheres.
Sequence analysis of proteins that were released into the supernatant, following sphere preparation by treatment with 4 m LiCl, was performed by LC-MS/MS.
However, the main process for the preparation of hollow spheres generally requires removable templates such as monodispersed silica, polystyrene latex spheres, metal nanoparticles, gas bubbles, and polymer spheres followed by sequential adsorption of magnetic nanoparticles on the templates [13, 14, 15, 16, 17, 18].
The preparation of hollow spheres is quite simple and scalable without the need for lithography.
In the past, various methodologies have been adopted for the preparation of hollow spheres [8, 9, 10, 11, 12].
Recently, Lu and co-works have reported the preparation of hollow spheres through weak acid base interaction-induced assembly with the use of oleic acid a soft template and functional dihydroxybenzoic acid (DA) as precursor [22].
To date, the solvothermal method was developed for the preparation of magnetite spheres with strong magnetization through the hydrolysis and reduction of iron chloride in ethylene glycol at high temperatures.
This synthesis method simplified the preparation of silica@polymer spheres by eliminating the previous core-forming step, and could apply many water-soluble biopolymers containing –NH2 groups as the shell materials.
The values normalized to Gapdh expression represent mean ±S.E.M. from three independent sphere preparations.
Results are expressed as Log2 ratios of values normalized to Gapdh and represent mean values ±S.E.M. from at least three independent sphere preparations.
