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For the sponge preparation, silk fibroin from the Bombyx mori silkworm and chitosan were used, in different proportions, as composites.
For sample preparation, silk fibroin (SF /hyaluronic acid (HA) scaffolds in different ratios of SF/HA (w/w) (i.e., 100 0, 90:10, 80 20, and 70 30) were formed by freeze-drying.
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Degummed silk fibers were used to prepare silk fibroin solution.
For the preparation of silk scaffolding, 600 μl of 2 wt.% silk fibroin solution was deposited on the coverslip surface at 60°C.
After the preparation of silk fibroin scaffolds by the technique of freeze drying, the scaffolds were pre-incubated in complete culture medium for 2 d, 6 d or 10 d before apical papilla stem cells (SCAP) seeding.
Pushpa, A., Vishnu, B. V. G. & K.S, T. R. Preparation of nano silk sericin based hydrogels from silk industry waste.
The preparation of patterned silk fibroin mold, consisting of the drop-casting of silk fibroin solution and the formation of silk nanostructure film replicated from patterned PDMS film, was a simple and practical process.
Teramoto, H., Nakajima, K. & Takabayashi, C. Preparation of elastic silk sericin hydrogel.
We discuss the implications of these findings for the preparation of regenerated silk for different applications.
This work suggests that such a controllable technique may help in the preparation of animal silk based materials with promising properties for various applications.
Here, we report preparation of anisotropic silk fibroin cryogels and scaffolds exhibiting a Young's modulus in the range of MPa that sustain up to 20 MPa compressive stresses.
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