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In preparation section, Prepare Protein module in Discovery Studio 2.5 (DS 2.5) was employed to protonate the X-ray crystallography structure of TRAF6 protein with Chemistry at HARvard Macromolecular Mechanics (CHARMM) force field [ 39] and remove crystal water.
The "recommended uses" include "properly prepared concrete," and the surface preparation section says, "The surface must be clean, dry and free of all loose or peeling paint," which implies it can be used over existing paint.
Samples were prepared as described in 'sample preparation' section.
In order to verify the advantage of suspension digestion, dough was prepared according to the 'Sample preparation' section.
In preparation section, the PKM2 proteins were prepared by pdb2gmx protocol of Gromacs to provide topology and parameters with CHARMM27 force field, and each ligand was prepared by SwissParam program [ 28] to provide topology and parameters with CHARMM.
See the preparation section for more information on preparing aquarium water.
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Tissues were prepared for histopathology (paraffin preparation, sectioning, and hematoxylin and eosin staining) and analyzed by subsequent microscopic evaluation by IDEXX Veterinary Services (West Sacramento, CA, USA).
Reactor Feed Preparation and Separator Feed Preparation Sections.
For in situ hybridization experiments, tissue preparation, sectioning and transcript detection were performed as described by Lozano et al. [79].
Standard stereological sample preparation, sectioning, and imaging of mouse lung tissues were performed for semi-automated acquisition of mean linear intercept (Lm) data.
In addition to the standard H&E preparation, sections were examined with special stains: Giemsa, Gram-Twort, Perls' Prussian Blue, Periodic Acid-Schiff, and Ziehl-Neelsen.
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