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For the experiments prepared in the absence of CO2, preparation, sampling, and reagent storage were done in a nitrogen atmosphere glove box.
Conventional adhesive preparation, sampling, protein electrophoresis, and then transferring to the membrane were done.
Culture preparation, sampling, and amendments were performed using strict anaerobic techniques based on the methods described by Miller and Wolin (1974).
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Sample preparation: samples were prepared as described in (Durfee et al., 2008) with modification as described here.
This combination facilitates automated reagent preparation, sample incubation, and sample purification for microsome stability studies.
After preparation, samples were examined by a Hitachi S-3000 scanning electron microscope (Chang et al. 2005).
When Huntington started her lab last year, she organized it into workstations: sample preparation, sample analysis, and general computing.
In analytical method development, method attributes are generally defined as the steps the method must have, such as sample preparation, sample introduction, sample analysis, and data analysis.
SJ helped with the preparation samples.
A human kidney preparation sample was used as control.
For the grid preparation sample was diluted to 25 µg/ml.
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